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monoclonal antibodies against pol ii ctd phospho s5 (h14  (Covance)

 
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    Structured Review

    Covance monoclonal antibodies against pol ii ctd phospho s5 (h14
    Monoclonal Antibodies Against Pol Ii Ctd Phospho S5 (H14, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h14+antibody/mouse+anti+pol+ii+ser5/pmc03127986-169-0-24
    Average 90 stars, based on 1 article reviews
    monoclonal antibodies against pol ii ctd phospho s5 (h14 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes
    Article Snippet: Chromatin was prepared from HCHO cross-linked cells, cultured as indicated in the figure legends, and immunoprecipitated using the following antibodies: anti-myc (Roche) for myc-tagged proteins, H14 (Covance) for Ser5-phosphorylated Rpb1, and anti-Rpb3 (Neoclone); following which the extracted DNA was subjected to PCR using the primers in Table S2 , as described in Supplementary Information .

    Article Title: The Lawc protein is required for proper transcription by RNA polymerase II in Drosophila
    Article Snippet: The following antibody dilutions were used: 1:1,000 rabbit α-Lawc, 1:5,000 rabbit α-Lamin, 1:500 H5 (Covance), 1:500 H14 (Covance), and 1:10,000 for all secondary antibodies (HRP α-rabbit and HRP α-mouse IgM).

    Article Title: Analysis of Drosophila p8 and p52 mutants reveals distinct roles for the maintenance of TFIIH stability and male germ cell differentiation
    Article Snippet: Primary antibodies used were: 8WG16 (1 : 1500; Covance), H14 (1 : 1500; Covance), XPD (1 : 1500; our own preparation), XPB (1 : 2000; our own preparation), p52 (1 : 1500 [ ]), p8 (1 : 1000 [ ]), Cdk7 (1 : 1000, Santa Cruz Biotechnology), TBP (1 : 500; Santa Cruz Biotechnology), E7 (1 : 2000; DSHB), A12 (1 : 1500; DSHB), F2F4 (1500; DSHB), JLA20 (1 : 3000; DSHB), GFP (1 : 2500; GenScript); p18 (1 : 1000; [ ]) HRP-coupled secondary antibodies (1 : 3500; Invitrogen) were used for chemioluminiscence detection with Thermo Scientific Pierce ECL.

    Article Title: TFIIH localization is highly dynamic during zygotic genome activation in Drosophila , and its depletion causes catastrophic mitosis.
    Article Snippet: Immunostainings were performed by standard methods with the following antibodies: p8 (1:100; Herrera-Cruz et al., 2012), H3S10P (1:1500; Santa Cruz Biotechnology), AA4.3 (1:800; DSHB), ADL67.1 (1:600; DSHB), H14 (1:500; Covance), TBP (1:100; Santa Cruz Biotechnology), CTD4H8 (1:100; Santa Cruz Biotechnology), GTU-88 (1:500; Abcam), CycH (1:500; Santa Cruz Biotechnology) and Alexa-Fluor dyes (Invitrogen).

    Incubation:

    Article Title: Histone Variant H2A.Z and RNA Polymerase II Transcription Elongation
    Article Snippet: .. One milliliter of chromatin solution containing approximately 1 mg/ml of protein was incubated with 8WG16 antibody or H14 antibody (Covance) overnight at 4°C. .. For Ser2-P, 10 l of H5 antibody (Covance) was preincubated overnight with 40 l of magnetic beads coupled to a goat anti-IgM (Dynal), washed with phosphate-buffered saline (PBS), and then added to the chromatin solution overnight.

    Article Title: The yeast regulator of transcription protein Rtr1 lacks an active site and phosphatase activity
    Article Snippet: .. Reactions were incubated for 1 hour at 30 °C, stopped by adding 5 μl 5X SDS loading buffer and 2.5 μl from each reaction was resolved on an 8% SDS-PAGE gel. pSer5 level was detected by Western blot using H14 antibody (Covance). ..

    SDS Page:

    Article Title: The yeast regulator of transcription protein Rtr1 lacks an active site and phosphatase activity
    Article Snippet: .. Reactions were incubated for 1 hour at 30 °C, stopped by adding 5 μl 5X SDS loading buffer and 2.5 μl from each reaction was resolved on an 8% SDS-PAGE gel. pSer5 level was detected by Western blot using H14 antibody (Covance). ..

    Western Blot:

    Article Title: The yeast regulator of transcription protein Rtr1 lacks an active site and phosphatase activity
    Article Snippet: .. Reactions were incubated for 1 hour at 30 °C, stopped by adding 5 μl 5X SDS loading buffer and 2.5 μl from each reaction was resolved on an 8% SDS-PAGE gel. pSer5 level was detected by Western blot using H14 antibody (Covance). ..

    Immunoprecipitation:

    Article Title: Identification of functional cis -regulatory elements by sequential enrichment from a randomized synthetic DNA library
    Article Snippet: .. After diluting the chromatin to 0.1% SDS, chromatin-Pol-II complexes were immunoprecipitated using the H14 antibody (Covance) specific to the Ser-5 phosphorylated form of Pol-II. ..

    Immunofluorescence:

    Article Title: Fcp1 Dephosphorylation of the RNA Polymerase II C-Terminal Domain Is Required for Efficient Transcription of Heat Shock Genes
    Article Snippet: .. Immunofluorescence of proteins on fixed polytene chromosomes was performed as described by Schwartz et al. ( 42 ), utilizing 1:50 H14 antibody (Covance), 1:25 Fcp1 antibody, 1:25 Scp1 antibody, and 1:20 Ssu72 antibody. ..



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